Project 2.1A: Cellular Reprogramming: Isolation and characterisation of adult, fetal and embryonic pluripotent cells in bovine
Project Leader: Dr Paul Verma - Monash Institute of Medical Research
AIMS:
- Isolation of bESC lines
- Isolation of pluripotent cells (HD) from bovine tissues
- Characterize pluripotent cell populations
PROGRESS 2004-05:
Research was conducted on methodologies for developing and analysing cell lines to get better outcomes for in vitro fertilisation and cloned embryos. Two independent approaches were explored. A project focused on chromatin remodelling involved analysis of gene expression data in all stages of pre-implantation IVP embryo development has demonstrated which genes are active at which phases of development. A second project on cell reprogramming has developed a new method of isolation and maintenance of bovine ES-like cells, and protected by a patent. Results from this research have many implications for the application of advanced breeding technologies in dairy cattle and other livestock. Advanced stem cell technologies offer the opportunity to improve performance of IVF-based animal breeding systems. In the dairy industry, this can help to shorten the interval between generations of animals and speed up the entire process of genetic improvement. The CRC is continuing this research in conjunction with the Australian Stem Cell Centre.
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